rabbit polyclonal anti mouse ap2  (Cell Signaling Technology Inc)

Journal: The Journal of biological chemistry

Article Title: Basic fibroblast growth factor decreases elastin gene transcription through an AP1/cAMP-response element hybrid site in the distal promoter.

doi: 10.1074/jbc.274.47.33433

Figure Lengend Snippet: FIG. 1. Gel shift analyses of elastin promoter restriction frag- ment and AP1 and AP2 consensus sequences with nuclear ex- tract from control and bFGF-treated pulmonary fibroblast cul- tures. Ten micrograms of nuclear proteins isolated from untreated and bFGF-treated pulmonary fibroblasts and 5.0 mg of poly(dI-dC) were incubated with 32P-labeled 2721- to 2472-bp restriction fragment or with 32P-labeled double-stranded AP1 or AP2 consensus oligode- oxynucleotides as described under “Materials and Methods.” The re- sulting complexes were resolved by electrophoresis through a 4% native polyacrylamide gel, and the DNA-protein complexes were visualized by autoradiography. Also shown in the 1st lane is the 32P-labeled 2721- to 2472-bp restriction fragment run without the addition of any nuclear extract as a control.

Article Snippet: Fra 1 (SC-183X), c-Jun (SC-45X), SP1 (SC-59X), and AP2 (SC-184X) rabbit polyclonal antibodies and horseradish peroxidaseconjugated anti-rabbit IgG (SC-2004) and anti-mouse IgG (SC-2005) were purchased from Santa Cruz Biotechnology.

Techniques: Gel Shift, Control, Isolation, Incubation, Labeling, Electrophoresis, Autoradiography